TB-500 Research Information
Synthetic Thymosin Beta-4 Fragment
For Tissue Repair & Regenerative Research
โ ๏ธ Important: This information is for educational and research purposes only. Always consult with a qualified healthcare professional before use.
๐ Contents
๐ฌ What is TB-500?
TB-500 is a synthetic peptide derived from thymosin beta-4 (Tฮฒ4), a naturally occurring protein found in human muscles that plays a vital role in tissue repair processes. It is a 17-amino acid peptide fragment (Ac-LKKTETQ) that encapsulates the primary biological activity of Tฮฒ4, acting as a potent regulator of cellular machinery essential for tissue repair.
TB-500 is available in pen and freeze-dried vial formulations for research purposes.
โก How TB-500 Works
TB-500's primary biological effect is the promotion of actin polymerization, a fundamental cellular protein crucial for cell shape, motility, and tissue remodeling.
Actin Regulation
Binds to G-actin, promoting cell migration, proliferation, and tissue repair
Angiogenesis
Stimulates VEGF signaling and new blood vessel formation
Anti-Inflammatory
Suppresses NF-ฮบB activation, reducing pro-inflammatory cytokines
๐ Research Applications
Muscle Regeneration
Accelerates recovery from muscle strains, reduces fibrosis, and improves functional outcomes
Tendon & Ligament Repair
Reduces post-surgical immobilization period and accelerates return to mobility
Neuroprotection
Attenuates neurite atrophy, inhibits microglial M1 polarization, and reduces neuroinflammation
Wound Healing
Promotes re-epithelialization and wound contraction in dermal models
๐ Clinical Studies
Phase 1 Safety Study (Wang et al., 2021)
A phase 1 safety study in healthy volunteers demonstrated that recombinant thymosin beta-4 was well tolerated at both single and multiple intravenous doses, with no dose-limiting toxicities or serious adverse events.
Phase 2 Dry Eye Disease Trial (Sosne et al., 2015)
Patients receiving thymosin beta-4 eye drops showed a 35% reduction in ocular discomfort and a 59% reduction in corneal staining compared to placebo.
Cardiology Pilot Study
A small pilot study using thymosin beta-4 to prime stem cells in patients with acute heart attacks showed improved exercise capacity and cardiac function at 6-month follow-up.
๐ Interactive Dosing Guide
Select your pen size and dose to see the corresponding volume, clicks, dosing frequency, and how many doses per pen:
๐๏ธ Step 1: Select Your Pen Size
๐ Step 2: Select Your Dose
Pen Size
10mg Pen
Selected Dose
1 mg
Doses per Pen
10 doses
Pen Clicks
30 clicks
Volume
0.30 mL
Protocol Level
Level 2 (Maintenance)
Recommended Schedule
Maintenance - 1-2 times per week
โ ๏ธ Do not change your dose unless advised by a qualified professional.
๐ Protocol Level Summary
TB-500 protocols are typically divided into a loading phase and a maintenance phase.
| Protocol Level | Target Dose | Frequency | Pen Clicks | Who It\'s For |
|---|---|---|---|---|
| Level 1 (Loading) | 1-2 mg | 1-2x daily | 30-60 clicks | Weeks 1-4, loading phase |
| Level 2 (Maintenance) | 2-5 mg | 1-2x weekly | 60-150 clicks | Week 5+, maintenance |
โ ๏ธ Do not change your dose unless advised by a qualified professional.
๐ Vial Users (Insulin Syringe)
Add 3mL of bacteriostatic water to your freeze-dried vial and mix gently. After reconstitution, use the table below to draw your dose:
Vial Size
10mg vial with 3mL bac water
| Dose | Frequency | Units on Syringe | Volume (mL) | Protocol Level |
|---|
โ ๏ธ Always use a new sterile needle and syringe for each injection.
โ ๏ธ Safety & Precautions
In human trials, TB-500 has exhibited good tolerability in the short term, with most adverse events being mild and transient. The phase 1 safety study reported no dose-limiting toxicities or serious adverse events.
Common side effects:
- Localized redness or swelling at injection site
- Occasional mild headache or fatigue
- Rare cases of allergic reaction
Do not use if you:
- Are allergic to TB-500 or any ingredients
- Are pregnant or breastfeeding
โ ๏ธ Warning: If you experience severe allergic reactions, difficulty breathing, or signs of anaphylaxis, seek immediate medical help.
๐ References
- TB-500 Research Overview. AMD Alliance. 2026.
- TB-500 Acetate: Mechanism of Action in Tissue Regeneration. Benchchem. 2025.
- TB-500 (10mg) Dosage Guide. Biohack Labs Help Center. 2026.
- Peng J. TB-500 for Injury Recovery: What the Research Actually Shows. Jeffrey Peng MD. 2026.
- Rahaman KA, et al. Simultaneous quantification of TB-500 and its metabolites. Journal of Chromatography B. 2024.
This information is for educational and research purposes only. Always consult with a qualified healthcare professional.
Last updated: July 2026